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KMID : 1007520120210061735
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Food Science and Biotechnology
2012 Volume.21 No. 6 p.1735 ~ p.1744
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Evidence for Protective Effects of Coffees on Oxidative Stressinduced Apoptosis through Antioxidant Capacity of Phenolics
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Lim Da-hee
Kim Woo-Ki
Lee Mi-Gi
Heo Ho-Jin
Chun Ock-K.
Kim Dae-Ok
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Abstract
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This study evaluated total phenolics, total flavonoids, and antioxidant capacity of randomly selected regular and decaffeinated coffees commercially available in Korea and their protective effects in human hepatic epithelial HepG2 cell line against oxidative stress. All coffees tested exhibited potent antioxidant capacity in chemical systems and, consequently, significant protection of cells from oxidative stress in vitro in a dose-dependent manner. In particular, H2O2-induced apoptosis as evaluated by annexin V staining and flow cytometry was prevented by coffee extracts, resulting in the enhanced cell viability. Of interest, the content of total phenolics and flavonoids in coffees demonstrated a positive correlation with antioxidant capacity, indicating that the antioxidant capacity of coffees may be attributed to those phytochemicals. In accordance with previous studies, caffeoylquinic acid (CQA) and its derivatives including 3-CQA, 4-CQA, 5-CQA, 3,4-diCQA, 3,5-diCQA, and 4,5-diCQA were identified as phenolic phytochemicals by a reversed-phase HPLC, with 5-CQA being a major component. Taken together, the present study demonstrated protective effects of regular and decaffeinated coffees on cells in vitro against overwhelming oxidative stress due to richness in phenolics, especially CQA and its derivatives. Coffees, regular or decaffeinated, may serve as a good source of health-beneficial phytochemicals in diet.
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KEYWORD
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anti-apoptosis, caffeoylquinic acid, flow cytometry, total phenolics, vitamin C equivale
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